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KAPA2G Fast HotStart PCR Kit

mutant from Thermus aquaticus, expressed in E. coli, plus 5x reaction buffer

For further processing only.

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Order information
KAPA2G Fast HotStart PCR Kit material number and pack size:
Material Number Pack Size
08041202001 5000 U
Will be supplied as "KAPA2G Fast HotStart PCR Kit". Unit of measure is "piece".
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Overview
Antibody-mediated hot start 2nd generation mutant of Taq, specifically designed for fast PCR.
The kit contains 2 vials of antibody-mediated hot start DNA polymerase and all buffers necessary to optimize the amplification reaction.
  • Save valuable time.
    Reach extension times as low as 1 sec/kb and reduce PCR reaction times by up to 75%.
  • Work with difficult templates
    Cover a broad range of both AT- and GC-rich targets.
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Application
Fast amplification of DNA fragments up to 3 kb in PCR assays:
  • Fast PCR
  • Routine PCR
  • Genotyping
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Specification
KAPA2G Fast HotStart DNA Polymerase is a second-generation enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq DNA Polymerase. In addition to speed, it provides higher yield and sensitivity than competitor enzymes across a broad range of targets.
Volume activity: 5 U/μL  
Unspecific endonucleases (plasmid DNA): Not detectable after 8 hours incubation at 37°C.
Exonucleases (λ DNA): Not detectable after 8 hours incubation at 37°C.
Tests for the presence of contaminating nucleic acids
(E. coli and related strains genomic DNA, 411 bp 16S rRNA fragment, <50 fg/μL): Corresponds to specification
(human genomic DNA, 290 bp b-actin fragment, <0.5 pg/μL): Corresponds to specification
Performance test (≥ 1 ng DNA): Corresponds to specification