• Broad spectrum

    of products for

    your industry

KAPA Probe Force

2x concentrated

For further processing only.

expand | collapse
+-
Order information
KAPA Probe Force material number and pack size:
Material Number Pack Size
08041237001 10 mL
08041229001 50 mL
08041237001: Will be supplied as "KAPA PROBE FORCE qPCR Master Mix, 10 mL". Unit of measure is "piece".
08041229001: Will be supplied as "KAPA PROBE FORCE qPCR Master Mix, 50 mL". Unit of measure is "piece".
+-
Overview
Antibody-mediated hot start DNA master mix containing a 3rd generation PCR inhibitor-resistant Taq mutant.
2x concentrated, ready-to-use antibody-mediated hot start PCR mix, containing KAPA3G DNA Polymerase in an optimized concentration for amplification of crude sample types. The master mix contains dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.
  • Easily work with crude samples and benefit from broad tolerance to carry-over inhibitors.
    Obtain accurate and reproducible results with direct PCR from crude blood, tissue and plant extracts.
  • Save valuable time and costs.
    Minimize the need for DNA purification and shorten your sample-to-result workflows to <1 hour.
  • Expand your options in assay development.
    Use for multiplexing qPCR applications with hydrolysis probe assays on a broad range of platforms.
+-
Application
 Use the qPCR master mix in application such as
  • Infectious disease testing
  • Cancer research
  • Food/water pathogen detection
  • SNP genotyping
  • GMO testing
  • Mouse transgenics
+-
Specification
KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-result workflows. The master mix contains a third generation (3G) DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility and sensitivity as purified DNA using KAPA PROBE FORCE.
Performance test in qPCR (mouse genomic DNA, β-2 microglobulin): Corresponds to specification
Tests for the presence of contaminating nucleic acids (bacterial genomic DNA <10 fg per standard 20 μL reaction): Corresponds to specification (human genomic DNA not detectable in standard 20 μL reaction): Corresponds to specification