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Anti-Digoxigenin

from mouse IgG1κ (clone 1.71.256), lyophilizate

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Order information
Anti-Digoxigenin material number and pack size:
Material Number Pack Size
11427458103 custom fill
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Overview
Monoclonal antibody to digoxigenin.
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Application
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
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Specification
Cross reactivity: There are no cross reactivities known.
Form of Supply: White lyophilized material
Molecular Weight: 150 to 170 kD, MW of digoxigenin is about 392.5 D.
Reconstitution: Add 1 mL double-distilled water to a final concentration of 100 μg/mL.
Storage Conditions:  -90 to -60°C, Antibody is stable at -90 to -60°C until expiration date printed on the label.
Working Concentration:
Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/mL
  • Immunohistocytochemistry: 0.5 to 2 μg/mL
  • In situ hybridization: 0.2 to 0.4 μg/mL
  • Western blot: 0.5 to 2 μg/mL