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Chlorophenolred-β-D-galactopyranoside (CPRG)

sodium salt, powder

For further processing only.

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Order information
Chlorophenolred-β-D-galactopyranoside (CPRG) material number and pack size:
Material Number Pack Size
11379119103 custom fill
Will be supplied as "Chlorophenolred-beta;-D-galactopyranoside (CPRG)".Unit of measure is "g".
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Application
Use CRPG as a substrate for β-Galactosidase.
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Specification
Formula: C25H21O10Cl2SNa
Molecular weight: 607.4 D
Identity (HPLC): Corresponds to specification
Appearance: Orange-red powder
Solubility: Clear, red colored solution in water (c=20 mg/mL)
A578 (c=5 mmol/L water) : ≤0.200
A650 (c=5 mmol/L water; turbidity): ≤0.030
Purity (HPLC, 265 nm): ≥97.5 area%
Purity (HPLC, 220 nm): ≥88 area%
Carbinol (HPLC): ≤10 area%
Total (CPRG+Carbinol, HPLC): ≥98 area%
CPRG (A405): 80-110%
Na (flame photometric): 3-4%
Water (K. Fischer): ≤15%
Thin layer chromatography: Corresponds to reference
Chlorophenolred, free: ≤0.1%
Galactose, free (enzymatic): ≤5.0%
Reaction rate (β-galactosidase) of sample/CPRG standard: 80-120%
Reaction rate (β-galactosidase) of sample/2-NP-galactoside: ≥8.5%
Stability: At -15 to -25°C within specification range for 36 months. Store dry. Keep tightly closed.