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Poly Peroxidase (Poly POD), EIA Grade

from horseradish, lyophilizate

For further processing only.

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Order information
Poly Peroxidase (Poly POD), EIA Grade material number and pack size:
Material Number Pack Size
11578545103 custom fill
Will be supplied as "Peroxidase, Polymerized (MH)". Unit of measure is "mg active ingredient".
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Overview
Poly Peroxidase is lyophilized in 10 mmol/L potassium phosphate, 50 mmol/L NaCl, 1 mmol/L EDTA, pH 6.1and saccharose as stabilizer.
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Application
Poly Peroxidase (Poly POD) is a marker enzyme enabling peroxidation of reduced dyes in the indicator reaction producing a color, fluorimetric or luminescent derivative of the labeled molecule for further detection and quantification.
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Specification
Molecular weight: 0.8 ± 0.2 x 106 D (~ 20 POD-monomers)
Activation: Is accomplished by MHS (Maleimidohexanoyl-N-hydroxysuccinimide ester) ≥40 MH-groups per Poly POD (MH) are accessible for conjugation with sulfhydryl groups.
Appearance: Red-brown lyophilizate
Solubility: Clear, red-brown solution in water (c= 5 mg/mL)
Specific activity (+25°C, ABTS): ≥600 U/mg
MH-groups: ≥2 (mol MH/mol POD)
Stability: At -60 to -90°C within specification range for 48 months.