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NxtScript RT Incubation Buffer

5x concentrated

For further processing only.

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Order information
NxtScript RT Incubation Buffer material number and pack size:
Material Number Pack Size
11465376103 1 mL
Will be supplied as "NxtScript RT Incubation Buffer, 5x". Unit of measure is "piece".
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Overview
Incubation buffer for two-step RT-PCR and cDNA synthesis using NxtScript Reverse Transcriptase, dNTPs and RNase Inhibitor.
  • Simple reaction setup. Use this premixed, pH-adjusted and contamination-controlled buffer for fast and easy setup of highly reproducible experiments.
  • Achieve excellent results. Benefit from the highly stable NxtScript Reverse Transcriptase for the synthesis of cDNA using this buffer.
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Application
Use NxtScript Incubation Buffer in combination with NxtScript Reverse Transriptase for:
  • Two-step RT-PCR applications
  • Generation of cDNA
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Specification
Appearance: Clear, colorless solution
Nonspecific endonucleases (MWM II DNA): Not detectable in up to 20 µL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 µL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 µL after 4 hours incubation at +37°C.
Function test in 2-step RT-PCR (human total RNA, 9.56 kb dystrophin gene fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.