NxtScript RT Incubation Buffer
For further processing only.
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NxtScript RT Incubation Buffer material number and pack size: Material Number Pack Size 11465376103 1 mL
Incubation buffer for two-step RT-PCR and cDNA synthesis using NxtScript Reverse Transcriptase, dNTPs and RNase Inhibitor.
- Simple reaction setup. Use this premixed, pH-adjusted and contamination-controlled buffer for fast and easy setup of highly reproducible experiments.
- Achieve excellent results. Benefit from the highly stable NxtScript Reverse Transcriptase for the synthesis of cDNA using this buffer.
- Use NxtScript Incubation Buffer in combination with NxtScript Reverse Transriptase for:
- Two-step RT-PCR applications
- Generation of cDNA
Appearance: Clear, colorless solution
Nonspecific endonucleases (MWM II DNA): Not detectable in up to 20 µL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 µL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 µL after 4 hours incubation at +37°C.
Function test in 2-step RT-PCR (human total RNA, 9.56 kb dystrophin gene fragment): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.