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GC-RICH Solution

5x concentrated

For further processing only.

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Order information
GC-RICH Solution material number and pack size:
Material Number Pack Size
12161583103 custom fill (5x conc.)
05917158103 1 mL (5x conc.)
12161575103: Will be supplied as "GC-rich solution, MBF". Unit of measure is "piece".
12161583103: Will be supplied as "GC-rich solution". Unit of measure is "L".
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Overview
Additive for efficient amplification of difficult DNA templates as GC-rich targets up to 5 kb in the polymerase chain reaction (PCR).
  • Enhance performance with difficult targets. Use GC-RICH Solution to quickly optimize amplification of difficult templates.
  • Obtain reliable results. Rely on the high lot-to-lot consistency of this product, thoroughly tested for a consistent quality.
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Application
Use GC-RICH Solution in combination with PCR buffer to improve the amplification of GC-rich template DNA, GC-rich targets and repetitive sequences, as well as mixtures of nucleic acids with varying GC content.
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Specification
Appearance: Clear, colorless solution.
Unspecific endonucleases (λDNA and MWM III DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 20 μL after 1 hour incubation at +37°C.
Function test in PCR (200 ng human genomic DNA, 284 bp ApoE fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.