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Transcriptor RT Buffer

5x concentrated

For further processing only.

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Order information
Transcriptor RT Buffer material number and pack size:
Material Number Pack Size
03531325103 1 mL (5x conc.)
Will be supplied as "Transcriptor RT Buffer". Unit of measure is "piece".
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Overview
Standard reaction buffer for Transcriptor Reverse Transcriptase.
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Application
Use Transcriptor RT Buffer as an optimized reaction buffer for Transcriptor Reverse Transcriptase for synthesis of cDNA from total RNA or mRNA.
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Specification
Appearance: Clear, colorless solution
Contents: Tris/HCl, 250 mmol/L; KCl, 150 mmol/L; MgCl2, 40 mmol/L; pH approximately 8.5 at +25°C
Unspecific endonucleases (MWM III DNA): Not detectable after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable after 4 hours incubation at +37°C.
Function test in 2-step RT-PCR (human total RNA, 10 kb dystrophin gene fragment): Corresponds to reference
Function test in real-time RT-qPCR using the LightCycler® Instrument (RNA standards, PBGD gene fragment): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.