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Dephosphorylation Buffer for Alkaline Phosphatase

10x concentrated

For further processing only.

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Order information
Dephosphorylation Buffer for Alkaline Phosphatase material number and pack size:
Material Number Pack Size
05989639103 1 mL
Will be supplied as ''Dephosphorylation Buffer, AP (10x), 1 mL''. Unit of measure is ''piece''.
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Overview
Standard reaction buffer for Alkaline Phosphatase.
  • Simplify reaction setup. Use this premixed, pH-adjusted and contamination-controlled standard reaction buffer for fast and easy setup of highly reproducible experiments.
  • Gain excellent performance. Take full advantage of this Dephosphorylation Buffer for Alkaline Phosphatase, using it as reaction buffer specially optimized for Alkaline Phosphatase, recombinant, 1 U/μL.
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Application
Use Dephosphorylation Buffer for Alkaline Phosphatase as optimized reaction buffer for Alkaline Phosphatase, recombinant, 1 U/μL.
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Specification
Appearance: Clear, colorless solution
Contents: 10x concentrated solution, comprising Tris/HCl, 500 mmol/L; EDTA, 1 mmol/L; pH approximately 8.5 at +20°C
Unspecific endonucleases (λDNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +37°C.
Ribonucleases (MWM II RNA): Not detectable in up to 20 μL after 1 hour incubation at +50°C.
Performance test (5' labeling on MWM III DNA, incorporation rate ≥30%): Corresponds to reference
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.