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α-S-dCTP, Molecular Diagnostic Grade

S-isomer, sodium salt, 100 mM

For further processing only.

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Order information
α-S-dCTP, Molecular Diagnostic Grade material number and pack size:
Material Number Pack Size
12207095103 100 mL (10000 µmol)
Will be supplied as "α-thio-d-CTP, Solution (Mol-DIA)". Unit of measure is "μmol".
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Overview
Modified dCTP for Strand Displacement Amplification and other special applications.
α-S-dCTP, Molecular Diagnostic Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice.
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Application
Use α-S-dCTP in alternative amplification technologies such as Strand Displacement Amplification (SDA). In addition, these nucleotides can be used in applications, including amplification reactions when a higher resolution in capillary electrophoresis is desired. This product is the purified S-isomer, prepared using Roche's biocatalytical production process. Use of purified S-isomer eliminates the introduction of another isomer which does not participate in the amplification reaction as it is not a substrate for the polymerase.
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Specification
Nomenclature: α-Thio-2'-deoxy-cytidine-5'-triphosphate
Formula: C9H16N3O12SP3 x H2O
Molecular weight: 483.2 D
Appearance: Colorless solution
pH value: 8.1-8.5
α-S-d-CTP (1 μmol ≙ 9.6 A272 units): 90-100 mmol/L
α-S-d-CTP, S-Isomer (HPLC): ≥98.0 area%
α-S-d-CDP (HPLC): ≤1.5 area%
DNases/RNases: Negative
Nicking activity: Negative
A250/A260: 0.80-0.84
A280/A260: 0.95-1.00
A290/A260: 0.28-0.32
Stability: At -15 to -25°C within specification range for 12 months.