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NucleoMix (dUTP), PCR Grade

sodium salt, 40 mM (10 mmol/L each dNTP)

For further processing only.

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Order information
NucleoMix (dUTP), PCR Grade material number and pack size:
Material Number Pack Size
03186075103 100 mL
Will be supplied as "Nucleomix (10 mmol/L,with dUTP)". Unit of measure is "mL".
GMP-manufactured NucleoMixes from the leading manufacturer of nucleotides for outstanding, consistent performance in amplification reactions.
NucleoMix (dUTP), PGR Grade, is supplied in sealed and CO2-proof bottles to ensure a stable pH during shipment on dry ice. The manufacturing process is fully validated including the final filling step. The pH is adjusted to match conditions for amplification.
NucleoMix (dUTP), PCR Grade, is designed for amplification reactions where high-quality reagents are required, such as for in vitro diagnostics. The ready-to-use mix eliminates process steps. Avoid carryover contamination between PCRs, a significant source of false positives. To decontaminate PCR and RT-PCR reagent mixes, dUTP is incorporated in place of dTTP. Subsequent reactions should be treated with Uracil-DNA Glycosylase (UNG) before amplification to degrade dUTP-containing PCR products.
Appearance: Clear, colorless solution
pH value: 8.1-8.5
Concentration (sum, 1 μmol ≙ 11.0 A260 units, pH 7.0): 40-44 mmol/L
Identity (HPLC diode array detector):
dATP: 9-12 mmol/L
dCTP: 9-12 mmol/L
dGTP: 9-12 mmol/L
dUTP: 9-12 mmol/L
dNTP (HPLC, sum of 4 peaks): ≥99 area%
dNDP (HPLC, sum of 4 peaks): ≤0.9 area%
DNases/RNases: Negative
Nicking activity: Negative
Function test in PCR (RNA, human dystrophin gene): Corresponds to specification
Stability: At -15 to -25°C within specification range for 35 months.