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COT Human DNA, CGH Grade

from human male placenta DNA, enriched for repetitive sequences, solution

For further processing only.

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Order information
COT Human DNA, CGH Grade material number and pack size:
Material Number Pack Size
05111854103 custom fill
Will be supplied as "COT Human DNA, CGH Grade". Unit of measure is "mL".
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Overview
Obtain best results for in situ suppression (CISS) hybridization, DNA microarray application and many other hybridization applications. With the COT Human DNA, CGH Grade, reproducible and sensitive measurements of dsDNA concentration are possible.
The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS) such as SINEs (small interspersed repetitive elements, e.g., Alu-elements) and LINEs (large interspersed repetitive elements, e.g., L1-elements) are distributed ubiquitously throughout the genome. COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
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Application
Use the COT Human DNA in the following applications:
  • Nucleic acid labeling and detection
  • DNA microarray applications such as comparative genome hybridization and sequence capture
  • Complex hybridization of human nucleic acids like FISH

In microarray applications, COT Human DNA is used in hybridization solutions to block repetitive DNA sample sequences from nonspecific hybridizations. In filter and other hybridization techniques, COT Human DNA is also used in prehybridization solutions to inactivate nonspecific target binding sites.
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Specification
Appearance: Clear, colorless solution
COT Human DNA (A260, water, 1 AB=50 μg/mL): ≥1.0 mg/mL
Fluorometrical determination of concentration: 1.0-1.5 mg/mL
Y-Chromosom (recovered exclusively from male human placenta): Corresponds to specification
A260/A280: 1.6-2.0
Absence of HIV 1/2 and HCV/HBV: Corresponds to specification
Performance test using gel electrophoretic separation (4% agarose gel without RE cleavage): middle chain length: 50-300 bp
Comparable intensity to previous lot: Corresponds to specification
Stability: At -15 to -25°C within specification range for 18 months.