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COT Human DNA

from human placenta DNA, enriched for repetitive sequences, solution

For further processing only.

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Order information
COT Human DNA material number and pack size:
Material Number Pack Size
11582011103 custom fill
Will be supplied as "DNA,Cot-1,human". Unit of measure is "mg".
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Overview
For suppression of cross-hybridization to human repetitive sequences in filter and in situ hybridizations.
The COT fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS) such as SINEs (small interspersed repetitive elements, e.g., Alu-elements) and LINEs (large interspersed repetitive elements, e.g., L1-elements) are distributed ubiquitously throughout the genome. COT Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
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Application
COT Human DNA is used in chromosome in situ suppression (CISS) hybridization. Cosmid or YAC probes contain repetitive elements that result in monospecific hybridization signals distributed over the entire chromosome. To enable specific hybridization to the chromosomal target site, the probe is denatured together with an excess of unlabeled COT Human DNA as a competitor. COT Human DNA can be used to suppress nonspecific hybridization to human repetitive sequences in microarray analysis, and in filter and in situ hybridization experiments.
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Specification
Appearance: Clear, colorless solution
COT Human DNA (A260, water): 1.0-2.0 mg/mL
Y-Chromosom (recovered exclusively from male human placenta): Corresponds to specification
A260/A280: 1.6-2.0
Absence of HIV 1/2 and HCV/HBV: Corresponds to specification
Performance test using gel electrophoretic separation (4% agarose gel, without RE cleavage): middle chain length: 50-300 bp
Comparable intensity of Cot 1 DNA (2 µg and 3 µg) to masterlot (agarose gel electrophoresis after ethylbromide staining): Corresponds to specification
Stability: At -15 to -25°C within specification range for 18 months.