• Broad spectrum

    of products for

    your industry

AptaTaq Genotyping Master (Rox)

5x concentrated

For further processing only.

expand | collapse
+-
Order information
AptaTaq Genotyping Master (Rox) material number and pack size:
Material Number Pack Size
05955823103 10 mL (for up to 5000 reactions at 10 μL final reaction volume)
05890144103 custom fill
05955823103: Will be supplied as ''AptaTaq Genotyping Master (ROX), 10 mL''. Unit of measure is ''piece''.
05890144103: Will be supplied as ''AptaTaq Genotyping Master (ROX)''. Unit of measure is ''mL''.
+-
Overview
Reversible hot start DNA master mix without initial activation step for maximum stability combined with sensitivity and specificity; lyo ready formulation for preparation of dried amplification mixes.
AptaTaq DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for the amplification of difficult sample types, reaction buffer, and a dNTP mix with dUTP (for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase). The special Rox reference dye (FRET-ROX) enables you to run assays for all real-time PCR instruments in which Rox reference dye is required for quantitative analysis.
  • Reduce time to result.
    Save up to 15 minutes per run by omitting the initial activation step required by chemically modified hot start polymerases, and reduce cycling time with fast protocols.
  • Ready for robotics.
    Rely on the stability of the AptaTaq Genotyping Master mix for PCR automation. The viscosity of the master mix is optimized for accurate pipetting. The mix is stable during setup and on the stacker for more than 24 hours.
  • Gain flexibility.
    The 5x concentrated master mix enables you to vary reaction volume and sample input for outstanding results. Use AptaTaq Genotyping Master mix for all real-time PCR instruments requiring Rox normalization.
  • Benefit from high stability.
    Keep the master mix in the refrigerator for up to 4 weeks and profit from a quick setup without thawing first.
+-
Application
Use AptaTaq Genotyping Master (Rox) in genotyping or other applications on instruments requiring normalization with Rox. AptaTaq Genotyping Master (Rox) is optimized for high-throughput applications using low reaction volumes. The master mix can be dried-down without loss of performance.
+-
Specification
The PCR master mix is very stable and can be stored in the refrigerator (+2 to +8°C) for at least 4 weeks without loss of activity and performance. It is stable at room temperature for at least 2 days.
Appearance: Clear, slightly violet solution
Performance test in qPCR using ABI 7900 HT
(human genomic DNA, CycA fragment): Corresponds to specification
(human genomic DNA, β-globin fragment): Corresponds to specification
(human genomic DNA, ApoE fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 12 months.
+-
License disclaimer

5:Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.