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EagleTaq DNA Polymerase, 270 U/μL

from Thermus aquaticus, recombinant in E. coli, solution

For further processing only.

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EagleTaq DNA Polymerase, 270 U/μL material number and pack size:
Material Number Pack Size
05394643190 25 kU approx. 0.1 ml 270 U/μl
Will be supplied as "CMPNT EAGLETAQ 25000U HIGH CONC". Unit of measure is "piece".
The gold standard of hot start Taq DNA Polymerases for maximum sensitivity and specificity. The concentrated formulation is suitable for preparation of dried amplification mixes.
  • Obtain high specificity, sensivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
  • Obtain reliable results.
    Use the gold standard of hot start polymerases for robust reaction performance.
Use EagleTaq DNA polymerase, 270 U/μL, especially for
  • Set-up of PCR master mixtures, if high concentrated components are requested
  • Preparation of stabilized dried formulations of reaction mixtures
Further applications: See EagleTaq DNA Polymerase, 5 U/μL
EagleTaq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand-specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Heat-activation: 95°C for 10-15 minutes (assay-dependent; recommendation is 15 minutes for full activation)
pH Optimum: ≈ 9.0 (at +20°C)
Temperature optimum: ≈ +75°C
Half life at +95°C: ≈ 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; EDTA, 0.1 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50.0% (v/v); pH approximately 9.0 at +20°C
Volume activity: 229-311 U/μL
Identity (SDS-PAGE): Corresponds to standard
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.