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EagleTaq DNA Polymerase, 270 U/µL

from Thermus aquaticus, recombinant in E. coli, solution

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Order information
EagleTaq DNA Polymerase, 270 U/µL material number and pack size:
Material Number Pack Size
05394643190 25 kU (approx. 0.1 mL, 270 U/µL)
Will be supplied as "CMPNT EAGLETAQ 25000U HIGH CONC". Unit of measure is "piece".
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Overview
The gold standard of hot start Taq DNA Polymerases for maximum sensitivity and specificity. The concentrated formulation is suitable for preparation of dried amplification mixes.
  • Obtain high specificity, sensivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
  • Obtain reliable results.
    Use the gold standard of hot start polymerases for robust reaction performance.
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Application
Use EagleTaq DNA polymerase, 270 U/µL, especially for
  • Set-up of PCR master mixtures, if high concentrated components are requested
  • Preparation of stabilized dried formulations of reaction mixtures
Further applications: See EagleTaq DNA Polymerase, 5 U/µL
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Specification
EagleTaq DNA Polymerase is designed for hot start PCR and has to be heat-activated in the beginning of the reaction protocol.
Enzyme acivities: Highly processive 5'–3' DNA polymerase; double-strand-specific 5'-3' exonuclease; no 3'–5' exonuclease activity
Heat-activation: 95°C for 10-15 minutes (assay-dependent; recommendation is 15 minutes for full activation)
pH Optimum: ≈ 9.0 (at +20°C)
Temperature optimum: ≈ +75°C
Half life at +95°C: ≈ 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 µmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration)
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; EDTA, 0.1 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; Tween 20, 0.2% (v/v); glycerol, 50.0% (v/v); pH approximately 9.0 at +20°C
Volume activity: 229-311 U/µL
Identity (SDS-PAGE): Corresponds to standard
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.