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HawkZ05 Fast One-Step RT-PCR Master (Rox)

2.3x concentrated

For life science research only. Not for use in diagnostic procedures.

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Order information
HawkZ05 Fast One-Step RT-PCR Master (Rox) material number and pack size:
Material Number Pack Size
05987687190 1 kit, 5 mL plus manganese acetate
Will be supplied as "KIT 1-STEP MMX W/ROX 1 x 5 mL RUO". Unit of measure is "piece".
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Overview
Reversible hot start one-step RT-PCR master mix with fast RT-step and high stability.
HawkZ05 Fast One-Step RT-PCR Kit is supplied as a kit containing 1 vial of HawkZ05 Fast One-Step RT-PCR Master Mix and 1 vial of RMS Manganese Acetate (25 mM). The master mix contains a reference dye (FRET-ROX) to run assays on real-time PCR instruments which require Rox for quantitative analysis.
  • Be flexible. 
    HawkZ05 Fast One-Step RT-PCR Kit enables amplification of both RNA and DNA targets.
  • Experience high performance. 
    Achieve reliable amplification of your low-copy RNA targets due to high temperature reverse transcription at +60 to +65°C and improved RNA processivity.
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Application
Apply HawkZ05 Fast One-Step RT-PCR Kit for:
  • High-throughput quantitative gene expression analysis
  • Target detection and quantification
  • Detection of rare transcripts
  • Reverse transcription and amplification of RNA from limited samples
  • Instruments requiring normalization with Rox
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Specification
Appearance: Clear, colorless solution
Function test: Average CT value of positive controls tested is between 20 and 30 cycles using starting template of 1x104 copy pAW 109 per reaction. Average CT value of real-time PCR test is within ±2 cycles of the proven specification.
Stability: At -15 to -25°C within specification range for 12 months.
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License disclaimer

65:NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses for diagnostic applications may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA.