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EagleTaq DNA Polymerase, 5 U/μL

from Thermus aquaticus, expressed in E. coli, solution

For further processing only.

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Order information
EagleTaq DNA Polymerase, 5 U/μL material number and pack size:
Material Number Pack Size
05206944190 1 kU
05206952190 25 kU
05206928190 500 kU
05206944190: Will be supplied as ''CMPNT EAGLETAQ 1 KU, 5 U/uL 0.2 mL''. Unit of measure is ''piece''.
05206952190: Will be supplied as ''CMPNT EAGLETAQ 25 KU, 5 U/uL 5 mL''. Unit of measure is ''piece''.
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Overview
EagleTaq DNA Polymerase is a chemically modified hot start DNA polymerase.
  • Obtain high specificity, sensivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
  • Obtain reliable results.
    Use the gold standard of hot start polymerases for robust reaction performance.
     
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Application
Apply EagleTaq DNA Polymerase for:
  • Hot start activated amplification
  • Incorporation of modified nucleotides for generating labeled PCR products
  • Detection formats such as hydrolysis probes, hybridization probes and SYBR Green
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Specification
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; EDTA, 0.1 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; Tween 20, 0.5% (v/v); glycerol, 50.0% (v/v); pH approximately 9.0 at +20°C
Volume activity: 5.4-5.9 U/μL
Function test: At least 1.5x105 fold amplification of λDNA after 25 cycles. One band at 500 bp on an agarose gel.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.
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License disclaimer

64:NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only.  Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

63:NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.