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EagleTaq DNA Polymerase, 5 U/μL

from Thermus aquaticus, expressed in E. coli, solution

For further processing only.

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Order information
EagleTaq DNA Polymerase, 5 U/μL material number and pack size:
Material Number Pack Size
05206944190 1 kU
05206952190 25 kU
05206928190 500 kU
05206944190: Will be supplied as ''CMPNT EAGLETAQ 1 KU, 5 U/uL 0.2 mL''. Unit of measure is ''piece''.
05206952190: Will be supplied as ''CMPNT EAGLETAQ 25 KU, 5 U/uL 5 mL''. Unit of measure is ''piece''.
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Overview
Hot start Taq DNA Polymerase for highly specific and sensitive amplification using PCR.
  • Obtain high specificity, sensivity, and yield.
    Prevent the extension of non-specifically bound primers using this hot start enzyme.
  • Obtain reliable results.
    Use the gold standard of hot start polymerases for robust reaction performance.
     
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Application
Apply EagleTaq DNA Polymerase for:
  • Hot start activated amplification
  • Incorporation of modified nucleotides for generating labeled PCR products
  • Detection formats such as hydrolysis probes, hybridization probes and SYBR Green
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Specification
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; EDTA, 0.1 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; Tween 20, 0.5% (v/v); glycerol, 50.0% (v/v); pH approximately 9.0 at +20°C
Volume activity: 5.4-5.9 U/μL
Function test: At least 1.5x105 fold amplification of λDNA after 25 cycles. One band at 500 bp on an agarose gel.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.