Taq DNA Polymerase, GMP Grade, 5 U/μl
from Thermus aquaticus BM, expressed in E. coli, solution
For further processing only.
Taq DNA Polymerase, GMP Grade, 5 U/μl material number and pack size: Material Number Pack Size 03707610103 1 kU (5 U/μL) 03707628103 5 kU (5 U/μL) 03161455103 50 kU (5 U/μL)
03707628103: Will be supplied as "Taq DNA Polymerase Ind. GMP Grade, 5 kU". Unit of measure is "piece".
03161455103: Will be supplied as "Taq DNA Polym GMP Grade 50ku". Unit of measure is "piece".
The enzyme is supplied without reaction buffer.
Taq DNA Polymerase is the robust standard enzyme for the amplification of DNA fragments up to 3 kb in PCR.
- Obtain consistent results.
Rely on the robust reaction performance and the lot-to-lot consistency of this product.
- Stay ahead of regulatory requirements.
High quality manufacturing, quality control and documentation according to GMP (Good Manufacturing Practice) regulations.
- Obtain consistent results.
- Use Taq DNA Polymerase, GMP Grade, 5 U/μl, for:
- Routine PCR and RT-PCR applications
- Amplification of DNA fragments up to 3 kb from various sources of DNA
- Labeling of DNA with modified nucleotides (e.g., DIG-dUTP, biotin-dUTP, fluorescein-dUTP)
- Combination with dUTP and Uracil-DNA Glycosylase for prevention of carryover contamination between PCR reactions
- Manufacture of amplification mixtures for applications with regulatory requirements (e.g.,in vitro diagnostics, quality control)
Taq DNA Polymerase is the recombinant full-length version of the thermostable enzyme from the eubacterium Thermus aquaticus BM, expressed in E. coli.
Enzyme acivities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
pH optimum: Approximately 9.0 (+20°C)
Temperature optimum: Approximately +75°C
Half life at +95°C: Approximately 40 minutes
Substrates: Incorporates dNTP, dUPT, dITP, various labeled or modified nucleotides (200 μmol/L each is recommended of normal dNTP, increased concentrations of variants)
Divalent ion requirement: Mg2+ (1.5 mmol/L standard concentration) Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 20 mmol/L; KCl, 100 mmol/L; DTT, 1 mmol/L; EDTA, 0.1 mmol/L; Nonidet P40, 0.5% (v/v); Tween 20, 0.5% (v/v); glycerol, 50% (v/v); pH approximately 8.0 at +4°C.
Volume activity: ≥5 U/μL
Specific activity (Protein: A280): ≥130,000 U/mg
Unit definition: One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleosidetriphosphates into acid precipitable DNA within 30 minutes at +75°C under standard assay conditions.
Purity (SDS PAGE): ≥98%
Unspecific endonucleases (λDNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 30 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 10 U after 1 hour incubation at +37°C.
Function test in PCR (10 pg λDNA, 0.5 kb fragment): Corresponds to reference
Function test in qPCR using LightCycler®
(human genomic DNA, β-globin gene): Corresponds to reference
(plasmid DNA, β-globin gene): Corresponds to reference
Bioburden: ≤50 CFU/mL
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.
48:Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.