Expand Long Template PCR Buffer 2
10x conc., with 27.5 mM MgCl2
For further processing only.
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Expand Long Template PCR Buffer 2 material number and pack size: Material Number Pack Size 05420075103 1 mL (10x conc., with 27.5 mM MgCl2)
- Reaction buffer 2 for PCR of fragments from 12 to 15 kb using the Expand Long Template PCR System.
- Use this buffer together with Expand Long Template PCR System.
Appearance: Clear, colorless solution
Contents: Tris/HCl, 500 mmol/L; (NH4)2SO4, 160 mmol/L; MgCl2, 27.5 mmol/L; DMSO, 20%; Tween 20, 1%; pH approximately 9.2 at +25°C
Unspecific endonucleases (λDNA and MWM III DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Nicking activity (pBR322 DNA): Not detectable in up to 20 μL after 16 hours incubation at +65°C.
Function test in PCR (200 ng human genomic DNA, 12 kb tPA fragment): Corresponds to specification
Stability: At -15 to -25°C within specification range for 24 months.