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RNase A

from bovine pancreas, lyophilizate, powder

For further processing only.

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Order information
RNase A material number and pack size:
Material Number Pack Size
10154105103 custom fill
Will be supplied as "Ribonuclease A from Bovine Pancreas". Unit of measure is "g".
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Overview
Standard RNase A in lyophilized quality is an essential tool for applications requiring RNA-free DNA templates.
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Application
Use RNase A for isolation of genomic DNA. For this purpose, RNase A should be boiled before use.
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Specification
Molecular weight: 13.7 kD
Specificity: Pyrimidine-specific endoribonuclease that acts on single-stranded RNA
Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=1 mg/mL)
Activity: ≥50 U/mg
Unit definition: One unit produces a decrease in absorbance from A0 to A1 in 1 minute under assay conditions (Kunitz). A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Unspecific endonucleases (λDNA): Not detectable in up to 1 μg after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 1 μg after 4 hours incubation at +37°C.
Proteases (A ≤0.1, 15 minutes, +37°C): Corresponds to reference
Turbidity, according to Maniatis (A366 ≤0.100): Corresponds to reference
A280 (1 mg/mL water): 0.54-0.72
pH ≤5.0 treatment (≥3 hours): Corresponds to reference
Countries of origin: South Africa, Argentina, Australia, New Zealand, Uruguay or the United States
Stability: At +2 to +8°C within specification range for 24 months. Store dry.