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NxtScript Reverse Transcriptase, concentrate

mutant from Moloney Murine Leukemia Virus, expressed in E. coli

For further processing only.

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Order information
NxtScript Reverse Transcriptase, concentrate material number and pack size:
Material Number Pack Size
07051166103 custom fill
Will be supplied as "NxtScript RT, conc.". Unit of measure is "MU".
M-MLV Reverse Transcriptase mutant designed for high thermostability and comparable performance to the market leader.
  • Reverse transcribe difficult templates.
    The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).
  • Achieve higher yield.
    NxtScript RT lacks RNase H activity. This results in higher cDNA yields.
  • Stay specific.
    Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target.
Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:
  • Two-step RT-PCR applications
  • RT-PCR for detection of viral targets
  • RT-PCR for detection of mRNA targets, such as cancer biomarkers
  • TMA and NASBA nucleic acid amplification methods
  • Generation of full-length cDNA libraries
  • Rapid amplification of cDNA ends (RACE)
NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg2+
Appearance: Clear, colorless solution
Activity: ≥250 U/µL
Unit definition: One unit NxtScript Reverse Transcriptase is defined as the amount of enzyme which incorporates 1nmol [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.
Purity (HPLC): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.
Stability: At -15 to -25°C wihtin specification range for 12 months.