NxtScript Reverse Transcriptase, concentrate
mutant from Moloney Murine Leukemia Virus, expressed in E. coli
For further processing only.
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NxtScript Reverse Transcriptase, concentrate material number and pack size: Material Number Pack Size 07051166103 custom fill
NxtScript RT is a designed M-MLV reverse transcriptase mutated for higher thermostability and comparable performance to the market leader.
- Reverse transcribe difficult templates. The high thermostability of NxtScript allows reactions up to +60°C to overcome RNA secondary structures (e.g., in GC-rich templates).
- Achieve higher yield. NxtScript RT lacks RNase H activity. This results in higher cDNA yields.
- Stay specific. Make use of the wide temperature activity range of NxtScript and reverse transcribe at the temperature that is optimal for your RNA target.
- Use NxtScript RT for synthesis of cDNA from total RNA or mRNA for:
- Two-step RT-PCR applications.
- RT-PCR for detection of viral targets.
- RT-PCR for detection of mRNA targets, such as cancer biomarkers.
- TMA and NSBA nucleic acid amplification methods.
- Generation of full-length cDNA libraries.
- Rapid amplification of cDNA ends (RACE).
NxtScript reverse transcriptase is highly thermostable and allows higher temperatures for reverse transcription, thus providing excellent results for difficult RNA targets.
Enzyme activities: RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, low RNase H activity, no endonuclease activity.
Recommended reaction temperature: +42 to +55°C
Substrates: Incorporates dNTP, ddNTP, dUTP, and various labeled or modified nucleotides.
Divalent ion requirement: Mg2+ Appearance: Clear, colorless solution
Activity: ≥250 U/µL
Unit definition: One unit NxtScript Reverse Transcriptase is defined as the amount of enzyme which incorporates 1nmol [3H]TMP into an acid insoluble product in 10 minutes at +37°C with poly(A)x(dT)15 as substrate.
Purity (HPLC): ≥90%
Unspecific endonucleases (MWM III DNA): Not detectable in up to 75 U after 16 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 150 U after 1 hour incubation at +37°C.
Stability: At -15 to -25°C wihtin specification range for 12 months.