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Alkaline Phosphatase, recombinant,
1 U/μL

from bovine intestine, expressed in Pichia pastoris, solution

For further processing only.

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Order information
Alkaline Phosphatase, recombinant,
1 U/μL material number and pack size:
Material Number Pack Size
04571550103 custom fill
Will be supplied as "Alkaline Phosphatase rec., MB Grade, 1 U". Unit of measure is "kU".
The enzyme is supplied without reaction buffer.
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Overview
Alkaline Phosphatase for 5' dephosphorylation of DNA and RNA.
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Application
Use Alkalkine Phosphatase, recombinant, 1 U/μL for:
  • Dephosphorylation of 5' phosphate from DNA and RNA
  • Coupling to other proteins via its amino or carbohydrate groups, for detection in immunoassays and western blot analysis. The increased heat instability of the recombinant enzyme allows quick deactivation by heating after dephosphorylation
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Specification
Enzyme activity: Alkaline Phosphatase, recombinant, 1 U/μL catalyzes the dephosphorylation of 5' phosphate from DNA and RNA.
pH activity optimum: 9.8
pH stability optimum: 8.0
Cofactor: Zn2+
Aktivators: Mg2+, Mn2+, Co2+
Inactivation: Complete inactivation after 5 minutes at +75°C.
Appearance: Clear, colorless solution
Storage buffer: Tris/HCl, 25 mmol/L; MgCl2, 1 mmol/L; ZnCl2, 0.1 mmol/L; glycerol, 50% (v/v), pH approximately 7.6 at +4°C
Volume activity: ≥1 U/μL
Specific activity: ≥5 kU/mg protein
Unit determination: Photometric test with 4-nitrophenyl phosphate
Unspecific endonucleases (λDNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Nicking activity (pBR322 DNA): Not detectable in up to 100 U after 4 hours incubation at +37°C.
Exonucleases (3H-DNA): Not detectable in up to 60 U after 4 hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 100 U after 1 hour incubation at +37°C.
Animal-derived additives: None
Stability: At -15 to -25°C within specification range for 24 months.