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Fluorescein-12-dUTP

1 mM solution

For further processing only.

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Order information
Fluorescein-12-dUTP material number and pack size:
Material Number Pack Size
11375601103 custom fill
Will be supplied as ''Fluorescein-12-dUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire.
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Overview
Enzymatic nonradioactive labeling reagent for cDNA synthesis, PCR, random primed labeling, nick-translation or primer extension.
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Application
Use Fluorescein-12-dUTP as a substrate for:
  • Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • Taq DNA polymerase
  • Reverse transcriptase (e.g., from AMV and M-MuLV)
Fluorescein-12-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling.
Fluorescein-labeled probes can be used for in situ hybridization with direct fluorescence detection and detection by ELISA using Anti-Fluorescein-AP, Fab fragments.
Repeated fluorescence labeling using Tetramethylrhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.
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Specification
Nomenclature: Fluorescein-12-deoxyuridine-5'-triphosphate
Formula: C39H37N4O21P3Li4
Molecular weight: 1018.4 D
Appearance: Clear, yellow solution
Fluorescein-12-dUTP (1 μmol ≙ 63.3 A495 units, phosphate buffer, 0.1 mol/L, pH 9.0): 1.0-1.1 mmol/L
Purity (HPLC, including isomere): 85.0-100.0 area%
Function test using In Situ Cell Death Detection Kit, Fluorescein (in situ hybridization): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.