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Biotin-16-dUTP

20 mM solution

For further processing only.

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Order information
Biotin-16-dUTP material number and pack size:
Material Number Pack Size
04889665103 custom fill
Will be supplied as "Biotin-16-dUTP, 20 mM". Unit of measure is "nmol".
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Overview
Biotin-labeled nucleotides are used for the efficient generation of biotinylated targets which can be subsequently captured using streptavidin coated solid phases or detected by streptavidin conjugates.
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Application
Use Biotin-16-dUTP for nonradioactive DNA labeling such as random priming, PCR labeling or nick translation.
Biotin-16-dUTP is used as a substrate for:
  • Terminal Transferase
  • DNA polymerase I (holoenzyme and Klenow fragment)
  • Taq DNA polymerase
  • Reverse Transcriptase (e.g., Transcriptor)
Biotin-16-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% Biotin-16-dUTP and 65% dTTP, as well as in PCR.
The nucleotide also serves as a substrate for Terminal Transferase in 3'-end labeling.
Biotin labeled DNA can be detected with:
  • Streptavidin-alkaline phosphatase conjugate and a chemiluminescent substrate (CSPD, CDP-Star) or a color substrate
  • Biotin Luminescence Detection Kit
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Specification
Nomenclature: Biotin-16-2'-deoxy-uridine-5'-triphosphate
Formula: C32H48N7O18P3SLi4
Molecular weight: 971.5 D
Appearance: Clear, colorless solution
Biotin-16-dUTP (1 μmol ≙ 10.7 A240 units, phosphate buffer, 0.1 mol/L, pH 7.0): 20-25 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test using Biotin-High Prime (0.3 pg): Corresponds to reference
Stability: At -15 to -25°C within specification range for 12 months.