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Anti-Digoxigenin-POD, Fab fragments

polyclonal antibodies from sheep, lyophilizate

For further processing only.

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Order information
Anti-Digoxigenin-POD, Fab fragments material number and pack size:
Material Number Pack Size
11210360103 custom fill
Will be supplied as "Anti-digoxigenin POD-conjugate". Unit of measure is "kU".
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Overview
Fab-fragments of a polyclonal anti-digoxigenin antibody conjugated to horseradish peroxidase for nonradioactive detection.
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Application
Use Anti-Digoxigenin-POD, Fab fragments, for the detection of digoxigenin-labeled compounds using:
  • Dot blots and ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot
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Specification
Molecular weight: Molecular weight is within a broad range of 200 kD to several million kD depending on how large the complexes are.
Specificity: Specific to digoxigenin and digoxin.
Cross-reactivity: None to other human estrogen or androgen steroids, e.g., estradiol or testosterone.
Appearance: Clear, brown solution
Volume activity (+25°C, ABTS): 3400-4600 U/mL
Storage buffer: Potassium phosphate 10 mmol/L; NaCl 100 mmol/L; succrose, 7.5%; RPLA4, 5 mg/mL; pH 7.5
pH 5.5 treatment (≥30 minutes): Corresponds to specification
Performance test (immune reaction signal height): Corresponds to specification
Stability: At -196°C within specification range for 48 months.