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Digoxigenin-11-UTP

3.5 mM solution

For further processing only.

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Order information
Digoxigenin-11-UTP material number and pack size:
Material Number Pack Size
03359239103 custom fill
Will be supplied as "Digoxigenin-11-UTP". Unit of measure is "nmol".
For other than the listed concentrations please inquire.
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Overview
Digoxigenin-11-UTP is used to label RNA with digoxigenine. DIG-labeling permits fast and sensitive detection without the need of radioactive material.
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Application
Use DIG-11-UTP as a substrate for SP6, T3, and T7 RNA polymerases. It replaces UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%. Linearized template DNA with T7, SP6, or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and DIG-11-UTP, respectively. Labeled RNA can be detected using Anti-Digoxigenin-AP or fab fragments.
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Specification
Nomenclature: Digoxigenin-11-uridine-5'-triphosphate
Formula: C43H61N4O22P3Li4
Molecular weight: 1106.7 D
pH stability: Digoxigenin is bound to the nucleotide portion via an alkali-resistant ether linkage. The preparation is stable to 0.1-0.5 M NaOH at +15 to +25°C.
Appearance: Clear, colorless solution
Digoxigenin-11-UTP (1 μmol ≙ 8.78 A290 units, phosphate buffer, 0.1 mol/L, pH 7.0): 3.3-3.7 mmol/L
Purity (HPLC): 85.0-100.0 area%
Stability: At -15 to -25°C within specification range for 18 months.