• Broad spectrum

    of products for

    your industry

Digoxigenin-11-dUTP, alkali-stable

1 mM solution

For further processing only.

expand | collapse
+-
Order information
Digoxigenin-11-dUTP, alkali-stable material number and pack size:
Material Number Pack Size
11093681103 custom fill
Will be supplied as ''DIG-11-dUTP''. Unit of measure is ''nmol''.
For other than the listed concentrations please inquire.
+-
Overview
Digoxigenin-11-dUTP, alkali-stable, replaces radioactive assays for even higher sensitivity. The required exposure time is drastically reduced compared to radioactive assays.
+-
Application
Use Digoxigenin-11-dUTP, alkali-stable, for nonradioactive DNA labeling (e.g., random priming, PCR labeling, tailing, or nick translation). DIG-11-dUTP replaces dTTP in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. It is ideal as a substrate for DNA polymerase,Taq DNA polymerase, Terminal Transferase and reverse transcriptase.
Note: For labeling of probes which are preferentially used in hybridization experiments where stripping and reprobing of the membrane is intended, use DIG-11-dUTP, alkali-labile.
+-
Specification
Nomenclature: Digoxigenin-11-2'-deoxy-uridine-5'-triphosphate
Formula: C43H61N4O21P3Li4
Molecular weight: 1090.7 D
Appearance: Clear, colorless solution
Digoxigenin-11-dUTP (1 μmol ≙ 8.78 A290 units, phosphate buffer, 0.1 mol/L, pH 7.0): 0.9-1.1 mmol/L
Purity (HPLC): 85.0-100.0 area%
Function test using DIG DNA Labeling and Detection Kit (down to 0.03 pg): Corresponds to reference
Stability: At -15 to -25°C within specification range for 18 months.