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NxtScript DNA Master

5x concentrated

For further processing only.

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Order information
NxtScript DNA Master material number and pack size:
Material Number Pack Size
07368143103 5 mL (5x conc.)
Will be supplied as "NxtScript DNA Master, 5x, 5 mL". Unit of measure is "piece".
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Overview
Reversible hot start DNA master mix for multiplexing and as DNA master mix component in RT-PCR.
NxtScript DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for multiplex qPCR or qRT-PCR. It uses aptamer-mediated reversible hot start technology for specific priming and fast PCR. The mix contains dNTP mix with dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.
  • Save time.
    Take advantage of the aptamer technology and run a fast PCR protocol.
  • Achieve high sensitivity.
    Detect low copy numbers of DNA or RNA targets with higher sensitivity using a 5x master mix concentration.
  • Ready for automation.
    Set up your reaction at room temperature.
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Application
Use NxtScript Reverse Transcriptase (07051166103) in combination with NxtScript DNA Master to run highly sensitive qRT-PCR reactions to detect RNA pathogens.
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Specification
NxtScript DNA Master is a sensitive and robust reaction mix for detection of DNA and RNA pathogens. It is stable at +2 to +8°C for 3 months and in its final reaction setup, for 4 hours at room temperature.
Appearance: Clear colorless solution
Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.