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NxtScript DNA Master

5x concentrated

For further processing only.

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Order information
NxtScript DNA Master material number and pack size:
Material Number Pack Size
07368143103 5 mL (5x conc.)
Will be supplied as "NxtScript DNA Master, 5x, 5 mL". Unit of measure is "piece".
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Overview
The NxtScript DNA Master is an easy-to-use 5x reaction mix optimized for multiplex qPCR or qRT-PCR of up to 5 targets. Use NxtScript Reverse Transcriptase (07051166103) in combination with NxtScript DNA Master to run highly sensitive qRT-PCR reactions to detect RNA pathogens.
NxtScript DNA Master is a 5x concentrated, ready-to-use, one component hot start PCR mix, containing AptaTaq DNA Polymerase in an optimized concentration for multiplex qPCR or qRT-PCR. It uses aptamer-mediated reversible hot start technology for specific priming and fast PCR. The mix contains dNTP mix with dUTP for prevention of DNA contamination by PCR carryover by pretreatment with Uracil-DNA Glycosylase.
  • Save time.
    Take advantage of the aptamer technology and run a fast PCR protocol.
  • Achieve high sensitivity.
    Detect low copy numbers of DNA or RNA targets with higher sensitivity using a 5x master mix concentration.
  • Ready for automation.
    Set up your reaction at room temperature.
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Application
Use NxtScript DNA Master for
  • DNA detection
  • RNA detection in combination with NxtScript Reverse Transcriptase (07051166103)
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Specification
NxtScript DNA Master is a sensitive and robust reaction mix for detection of DNA and RNA pathogens. It is stable at +2 to +8°C for 3 months and in its final reaction setup, for 4 hours at room temperature.
Appearance: Clear colorless solution
Function test in qPCR (human reference cDNA G6PDH/β2M fragments): Corresponds to reference
Stability: At -15 to -25°C within specification range for 15 months.
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License disclaimer

63:NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

80:NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by US Patent Nos. 5,670,637, 5,693,502, and corresponding patent claims outside the US.  No right under any other patent claim is conveyed expressly, by implication, or by estoppel.