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Liberase MTF C/T, GMP Grade

0.2 μm filtered, lyophilizate

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Order information
Liberase MTF C/T, GMP Grade material number and pack size:
Material Number Pack Size
05339880001 1 kit 2,000 U Collagenase I/II MTF, 2x
15 mg Thermolysin MTF, 3x
Will be supplied as "Liberase MTF C/T GMP Grade". Unit of measure is "piece".
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Overview
Liberase MTF C/T GMP Grade kit is a highly purified enzyme blend for tissue dissociation where high purity, consistent quality and low levels of bacterial endotoxins are required in achieving reproducible high cell yield and viability.
Liberase MTF C/T, GMP Grade kit contains highly purified collagenase class I and class II from Clostridium histolyticum. The two collagenase isoforms are blended in a precise ratio with each other. Highly purified thermolysin from Bacillus thermoproteolyticus is provided in a separate vial to allow individual adjustments of enzyme ratios for optimization of your tissue dissociation protocol.
  • Maximum cell yield, viability, and functionality
    Use of high-specific-activity blends with reduced clostripain, trypsin, and endotoxin content maximizes yield of viable, functional cells.
  • Reduced contamination risk
    Manufactured free of any mammalian- or avian-derived raw materials, avoiding the risk of transmission of animal diseases.
  • High experimental reproducibility
    High lot-to-lot consistency due to optimized enzyme fermentation and purification processes results in increased experimental reproducibility, ultimately saving time and resources.
  • Flexible enzyme portfolio
    Custom sizes and blends are available upon request to better serve your unique application needs.
  • Specific residual assay
    Highly sensitive assay developed specifically for detection of Liberase enzyme verifies removal from your final product.
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Application
Use the Liberase MTF C/T, GMP Grade kit for in vitro enzymatic dissociation of tissue, to isolate single cells. In particular intended for use in isolation procedures of pancreatic islets from human and pig.
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Specification
I. Collagenase 2000 MTF MPB
Appearance: White lyophilized cake
Protein/bottle (target: 537 mg protein): 430-644 mg
Collagenase I content (target 322 mg protein): 258-386 mg
Collagenase II content (target 215 mg protein): 172-258 mg
Collagenase I b, c part of Collagenase I: ≤10 area%
HPLC purity (total peak areas of Col I + Col II): ≥85%
Activity (Wünsch, calculated): 2,172-3,617 U/vial
Ratio collagenase II/total collagenase (HPLC): 0.3-0.5
Bioburden: ≤10 CFU/bottle
Endotoxins: ≤10 EU/mg protein

II. Thermolysin MTF MPB
Appearance
: White lyophilized cake
Protein mass/bottle (target: 15.0 mg protein): 12.0-18.0 mg
Activity neutral protease (calculated): 130,500-234,000 U/vial
HPLC purity (total peak area thermolysin): ≥85%
Bioburden: ≤10 CFU/bottle
Endotoxins: ≤50 EU/mg protein
Stability: At -15 to -25°C within specification range for 24 months.
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Performance
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