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Colipase

from porcine pancreas, lyophilizate

For further processing only.

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Order information
Colipase material number and pack size:
Material Number Pack Size
10204307103 custom fill
Will be supplied as "Colipase from Porcine Pancreas". Unit of measure is "g active ingredient".
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Application
Use Colipase as a co-emulsifier in diagnostic tests for the determination of lipase activity in combination with chromogenic Lipase Substrate, Catalog No. 11 034 618 103.
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Specification
Molecular weight: Approximately 10 kD
Isoelectric point: 5.0
pH optimum: 8.8 (see figure)
Temperature dependence: See figure
pH stability: 3.5-11.5 (see figure)
Thermal stability: +25 to +80°C (see figure)
Specificity: Pancreatic colipase consists of 3 forms, colipase101 (procolipase), colipase96 and colipase85 (numbers stand for amino acid residues). colipase96 and colipase85 are trypsin digestion products of colipase101.
Appearance: White lyophilizate, TEA buffer, pH 6.0
Solubility: Clear, colorless solution in water (c=1 mg/mL)
pH value (c=1 mg/mL in water): 5.0-7.0
Activity (+25°C, tributyrin): ≥70,000 U/mg lyophilizate
Colipase: ≥0.6 mg/mg lyophilizate
Protein (Lowry): 0.8±0.2 mg/mg lyophilizate
Contaminants:
Lipase: ≤0.0005 U/mg lyophilizate
Proteases: ≤180 U/mg lyophilizate
SVD free: Corresponds to specification
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 24 months. Store dry.
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Performance
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