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Peroxidase (POD), Grade II

from horse radish, lyophilizate

For further processing only.

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Order information
Peroxidase (POD), Grade II material number and pack size:
Material Number Pack Size
11378783103 custom fill
Will be supplied as "Peroxidase (POD), Grade II, Horse-radish". Unit of measure is "MU".
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Application
Use Peroxidase (POD), Grade II, for the oxidation of reduced dyes in the indicator reaction of many diagnostic tests, e.g., for the determination of blood glucose, triglycerides or lactate. It may also be used as a marker enzyme for enzyme immunoassays (EIA).
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Specification
Nomenclature: Donor:hydrogen-peroxide oxidoreductase
Molecular weight: 40 kD
Structure: Glycoprotein with one mole of protoheme IX
Isoelectric point: 7.2
Rate constants:
a) Hydrogen acceptors:
H2O2 9 x 108 [L x mol-1 x s-1]
methyl peroxide 1.5 x 106 [L x mol-1 x s-1]
ethyl peroxide 3.6 x 106 [L x mol-1 x s-1]
b) Hydrogen donors: Many
Inhibitors: Cyanide, sulfide, fluoride, azide, hydroxylamine, hydroxyl ions
Activators: Peroxidation of o-dianisidine is accelerated by ammonia, pyridine, imidazole at pH values >7.0
pH optimum: 6.0-6.5 (see figure)
Temperature dependence: See figure
pH stability: 4.0-10.0 (see figure)
Thermal stability: Up to +40°C (see figure)
Specificity: Peroxidase is specific for the hydrogen acceptor; only H2O2, methyl- and ethylperoxides are active. In contrast the enzyme is not specific for the hydrogen donor. A large number of phenols, aminophenols, diamines, indophenols, leucocyte dyes, ascorbate and several amino acids react.
Appearance: Red-brown lyophilizate
Solubility: Clear, red-brown solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 6.6-7.6
Activity: ≥200 U/mg lyophilizate
Purity number (A403/A275): 2.0-3.5
A500 (100 U/mL): ≤0.120
Contaminants (expressed as percentage of Peroxidase activity):
ATPase: ≤0.001
Catalase: ≤0.7
Contaminating oxidases: ≤0.00005
Phosphatase, acidic: ≤0.001
Glucose: ≤0.25 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 24 months. Store dry.
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Performance
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