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Pyrophosphatase, rec., GMP Grade

from yeast, expressed in E. coli, solution

For further processing only.

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Order information
Pyrophosphatase, rec., GMP Grade material number and pack size:
Material Number Pack Size
08140677103 20 mL
Unit of measure is "piece".
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Overview
Pyrophosphatase is an enzyme, which hydrolyzes inorganic pyrophosphate.
Use fit-for-purpose Pyrophosphatase for manufacturing of therapeutic mRNA:
  • Pyrophosphatase is produced in antibiotic-free and animal-free conditions
  • QC-release testing includes extended impurity testing e.g. host-cell DNA, host cell proteins, endotoxin, bioburden, heavy metals
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Application
Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side.
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Specification
Pyrophosphatase is expressed with an N-terminal His-Tag.
Molecular weight: 63 kD
Cofactor: Mg2+ is required ofor enzymatic activity
Isoelectric point: pH 4.8
Appearance: Clear, colorless to slightly yellowish solution
Volume activity: ≥ 600 U/mL
Protein (abs. 280nm): 1.8-2.2 mg/mL
Purity (SDS-PAGE): ≥ 95 %
Unspecific endonucleases (up to 300 ng enzyme using MWM III-DNA; 16 h/37°C): not detectable
Nicking activity (up to 300 ng enzyme using pBR 322-DNA) 16 h/37°C): not detectable
RNase activity (up to 300 ng enzyme using MS II-RNA; 1 h/37°C): not detectable
Proteases (50 μg/15 min/37°C): not detectable
Exonucleases (using 1 μg): not detectable
Endotoxins: ≤ 10 EU/mL
Bioburden: ≤ 10 CFU/mL
E. coli host cell DNA: ≤ 100 pg/mg Protein
E. coli host cell protein: ≤ 50 ppm
Heavy metals (Ni, Co, V): ≤ 10 ppm
Stability: At -15 to -25°C within specification range for 18 months.