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Sarcosine Oxidase

from E.coli overproducer, lyophilizate

For further processing only.

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Order information
Sarcosine Oxidase material number and pack size:
Material Number Pack Size
11378856103 custom fill
Will be supplied as "Sarcosine Oxidase, Recombinant (E. coli)". Unit of measure is "kU".
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Overview
Oxidoreductase that catalyzes the demethylation of sarcosine to glycine.
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Application
Use Sarcosine Oxidase in diagnostic tests for the determination of creatinine. This can be done using one of two methods:
(1) In combination with Creatinase, Catalog No. 11 799 142 103 and Creatininase, Catalog No. 11 865 471 103.
(2) In combination with Creatinine Deaminase, Catalog No. 11 330 764 103, N-Carbamoylsarcosine Amidase, Catalog No. 11 248 847 103 and N-Methylhydantoinase (ATP-hydrolyzing), Catalog No. 11 288 555 103.
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Specification
Nomenclature: Sarcosine:oxygen oxidoreductase (demethylating)
Molecular weight: 40 kD (PAGE, native Phast®-System)
Isoelectric point: 5.3 (Phast®-System)
Michaelis constants (Tris buffer, 0.1 mol/L, pH 8.0; Sarcosine):
at +25°C: 3.7 x 10-3 mol/L
at +37°C: 6.3 x 10-3 mol/L
Inhibitors: Completely inhibited by ZnCl2 (7 mmol/L), CdCl2 (7 mol/L), heavy metals and NaN3. Chloroacetic amine (0.2%) does not inhibit.
pH optimum: 8.0 (see figure)
Temperature dependence: See figure
pH stability: 7.0-10.0 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Sarcosine Oxidase reacts with sarcosine (100%), N-ethylglycine, 2 mmol/L (4%), L(-)-proline (0.28%), carbamoylsarcosine (0%), and glycine (0%).
Appearance: Yellow lyophilizate
Solubility: Clear, yellow solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 7.5-8.5
Activity (+25°C, sarcosine): 22-40 U/mg lyophilizate
Specific activity: ≥45 U/mg protein
Protein (Biuret): 0.4-0.6 mg/mg lyophilizate
Contaminants (expressed as percentage of Sarcosine Oxidase activity):
ATPase: ≤0.01
Catalase: ≤10.0
Contaminating oxidases (FOX): ≤0.005
Creatinase: ≤0.001
Creatininase: ≤0.01
Creatinine deaminase: ≤0.001
N-Carbamoylsarcosine amidohydrolase: ≤0.001
N-Methylhydantoin hydrolase: ≤0.001
Stability: At -15 to -25°C within specification range for 12 months. Store dry. Protect from light.
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Performance
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