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Cholesterol Esterase, chemically modified

from Pseudomonas species, lyophilizate

For further processing only.

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Order information
Cholesterol Esterase, chemically modified material number and pack size:
Material Number Pack Size
11641735103 custom fill
Will be supplied as "Cholesterol Esterase Modified". Unit of measure is "MU".
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Overview
Hydrolase that splits fatty acids from sterols.
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Application
Use Cholesterol Esterase, chemically modified in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 11 479 709 103, or 10 634 522 103.
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Specification
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure for product Cat. No. 11 520 857 103)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure for product Cat. No. 11 520 857 103)
Thermal stability: Below +20°C (see figure for product Cat. No. 11 520 857 103)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/mL)
Activity (+25°C, cholesterol oleate): ≥10 U/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤1.00
Glycerokinase: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.001
Uricase: ≤0.005
NaCl: 3±0.2 mol/L
Stability: At +2 to +8°C within specification range for 12 months. Store dry.
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Performance
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