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Cholesterol Esterase, Grade I

from Pseudomonas species, lyophilizate

For further processing only.

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Order information
Cholesterol Esterase, Grade I material number and pack size:
Material Number Pack Size
11520857103 custom fill
Will be supplied as "CE, Ps.species, Lyo., SQ". Unit of measure is "MU".
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Overview
Hydrolase that splits fatty acids from sterols.
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Application
Use Cholesterol Esterase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 10 634 522 103, or 11 479 709 103.
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Specification
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure)
Thermal stability: Below +20°C (see figure)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.
Remark: This Cholesterol esterase is especially suited for liquid stable applications with extended shelf life requirements.
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, cholesterol oleate): ≥100 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Protein (Biuret): No limit
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤1.00
Glycerokinase: ≤0.001
Glucose oxidase: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.001
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Store dry.
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Performance
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