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Cholesterol Esterase, Grade II

from Pseudomonas species, lyophilizate

For further processing only.

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Order information
Cholesterol Esterase, Grade II material number and pack size:
Material Number Pack Size
11015923103 custom fill
Will be supplied as "Cholesterol Esterase from Pseudom.species". Unit of measure is "MU".
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Overview
Hydrolase that splits fatty acids from sterols.
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Application
Use Cholesterol Esterase in diagnostic tests for the determination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos. 10 634 522 103 or 11 479 709 103.
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Specification
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10-5 mol/L
Inhibitors: Heavy metals such as Cu2+, Ag+, Zn2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure for product Cat. No. 11 520 857 103)
Temperature dependence: Not possible to determine under assay conditions due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure for product Cat. No. 11 520 857 103)
Thermal stability: Below +20°C (see figure for product Cat. No. 11 520 857 103)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives complete cleavage of all serum cholesterol esters.
Appearance: Slightly yellowish lyophilizate
Solubility: Clear, colorless or yellowish to brownish solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Ficoll (colorimetric): 40-80 mg/100 mg lyophilizate
Activity (+25°C, cholesterol oleate): ≥30 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Protein (Biuret): 0.1-0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤200 U/mg lyophilizate
Glycerokinase: ≤0.001
GOD: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.005
Uricase: ≤0.005
Stability: At +2 to +8°C within specification range for 12 months.