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Endoproteinase Lys-C, Sequencing Grade

from Lysobacter enzymogenes, lyophilizate

For further processing only.

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Order information
Endoproteinase Lys-C, Sequencing Grade material number and pack size:
Material Number Pack Size
11058533103 5 μg
Will be supplied as ''Endoprot. Lys-C, Sequ., MPB 5 UG''. Unit of measure is ''piece''.
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Overview
Endoproteinase Lys-C, Sequencing Grade, can be used for specific cleavage of peptides.
Endoproteinase Lys-C, Sequencing Grade, is a widely used serine protease, that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of lysin. Endoproteinase Lys-C is isolated from Lysobacter enzymogenes and is supplied as a lyophilizate.
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Application
Use Endoproteinase Lys-C, Sequencing Grade, for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.
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Specification
Molecular weight: 33 kD (reduced), 30 kD (nonreduced)
pH optimum: 8.5-8.8
Inhibitors: DFP, TLCK, aprotinin, and leupeptin
Appearance: White lyophilizate
Activity (+25°C, Chromozyme® PL): ≥1 U/bottle
Specific activity: ≥200 U/mg protein
Purity (SDS PAGE, homogeneity Phast-system® ; 8-25%): ≥90%
Specificity (HPLC, melittin, after 1 hour incubation): ≥90%
Unspecific cleavage products after 18 hours: ≤5%
Stability: At +2 to +8°C within specification range for 12 months. Store dry.