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Aspartate Aminotransferase (AST) (GOT)

from pig heart, suspension

For further processing only.

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Order information
Aspartate Aminotransferase (AST) (GOT) material number and pack size:
Material Number Pack Size
10153354103 custom fill
Will be supplied as "GOT from Pig Heart". Unit of measure is "MU".
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Application
Use Aspartate Aminotransferase for designing your calibrator/control reagent and for the synthesis of unnatural L-amino acids from α-keto acids.
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Specification
Appearance: Yellow suspension in ammonium sulfate
pH value: 5.5-6.5
Specific activity (+25°C; L-aspartate, α-ketoglutarate): ≥200 U/mg protein
Protein: ≥10 mg/ml (standardized to 10±1 mg/mL)
Ammonium sulfate: 3.2±0.2 mol/L
Contaminants (expressed as percentage of Aspartate Aminotransferase activity):
Glutamate dehydrogenase: ≤0.01
Alanine Aminotransferase (ALT/GPT): ≤0.01
Lactate dehydrogenase: ≤0.01
Malate dehydrogenase: ≤0.01
Oxaloacetate decarboxylase:≤0.01
SDV free: Corresponds
pH 5.5 treatment (30 minutes): Corresponds to specification
Stability: At +2 to +8°C within specification range for 24 months. Store dry.