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Papain

from Carica papaya, suspension

For further processing only.

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Order information
Papain material number and pack size:
Material Number Pack Size
10154644103 custom fill
Will be supplied as "Papain from Carica papaya". Unit of measure is "g".
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Overview
Papain can be used for complete proteolytic cleavage of peptides and proteins.
Papain is a cysteine endopeptidase, hydrolyzing proteins, peptides, amides, and esters of amino acids and peptides, especially at bonds involving Arg, Lys, Glu, His, Gly and Tyr. Upon prolonged incubation, further bonds are cleaved; shows also esterase and transamidase activity, and is used for peptide synthesis.
  • Increase the safety of your processes with reproducible performance and high lot-to-lot consistency.

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Application
Use Papain for:
  • Complete proteolytic cleavage of peptides and proteins
  • Limited hydrolysis of native immunoglobulins
  • Tissue dissociation (together with collagenase, esterase, trypsin)
  • Solubilization of integral membrane proteins
  • Production of glycopeptides from purified proteoglycans
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Specification
Molecular weight: 23 kD ±2
Appearance: White suspension
pH value (c=10 mg/mL): 4.0-5.0
Specific activity (+25°C, BAEE): ≥30 U/mg protein
Protein (A280, 1%=24): ≥10 mg/mL
Stability: At + 2 to +8°C within specification range for 12 months.