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Pyruvate Kinase

from Bacillus stearothermophilus, lyophilizate

For further processing only.

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Order information
Pyruvate Kinase material number and pack size:
Material Number Pack Size
11462652103 custom fill
Will be supplied as "PK, Bacillus stearothermoph., Lyo.". Unit of measure is "MU".
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Application
Use Pyruvate Kinase to catalyze the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, e.g., for the enzymatic determination of potassium or triglycerides.
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Specification
Appearance: White lyophilizate
Solubilty: Clear, colorless solution in water (c=10 mg/mL)
pH value (+25°C, c=10 mg/mL): 7.6-8.7
Activity (+37°C, PEP): ≥120 U/mg lyophilized material
Specific activity: ≥150 U/mg protein
Protein (Biuret): 0.55-0.95 mg/mg lyophilizate
Contaminants (expressed as percentage of Pyruvate Kinase activity):
''NADH oxidase'' (dA365, 48 hours): ≤0.060
Na (flame photometric): ≤30 μmol/KU
K (flame photometric): ≤0.6 μmol/KU
NH4 (enzymatic): ≤0.13 mg/KU
Mg (AAS): ≤50 μmol/KU
Mn (AAS): ≤2.4 μmol/KU
Stability: At +2 to +8°C within specification range for 12 months. Store dry.