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Pyruvate Kinase

from rabbit muscle, suspension

For further processing only.

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Order information
Pyruvate Kinase material number and pack size:
Material Number Pack Size
10005533103 custom fill
Will be supplied as "Pyruvate Kinase (PK) from Rabbit Muscle". Unit of measure is "MU".
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Application
Use Pyruvate Kinase to catalyze the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, e.g., for the enzymatic determination of potassium or triglycerides.
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Specification
Appearance: White suspension in ammonium sulfate, 3.2 mol/L, pH approximately 6
pH value: 5.5 - 6.5
Activity (+25°C, PEP): ≥2,000 U/mL solution
Specific activity: ≥200 U/mg protein
Protein (Biuret): ≥10 mg/mL
Ammonium sulfate: 3.2±0.2 mol/L
Contaminants (expressed as percentage of Pyruvate Kinase activity):
ATPase: ≤0.002
Enolase: ≤0.01
Glycerokinase: ≤0.001
Hexokinase: ≤0.002
Lactate dehydrogenase: ≤0.01
Myokinase: ≤0.01
''NADH oxidase'': ≤0.002
Glycerol (enzymatic): ≤10 μg/10 mg
Stability: At +2 to +8°C within specification range for 24 months.