• Broad spectrum

    of products for

    your industry

Glycerol-3-phosphate Oxidase, chemically modified

from E.coli overproducer, lyophilizate

For further processing only.

expand | collapse
+-
Order information
Glycerol-3-phosphate Oxidase, chemically modified material number and pack size:
Material Number Pack Size
11582003103 custom fill
Will be supplied as "L-Glycerol-3-phosphate Oxidase rec. mod.". Unit of measure is "MU".
Additional formulation: Lyophilizate, Catalog No. 11 654 730 103
+-
Overview
Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate.
+-
Application
Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase, Catalog Nos. 10 539 937 103 or 11 499 530 103 and Lipoprotein Lipase, Catalog No. 11 145 991 103.
+-
Specification
Nomenclature: Glycerol-3-phosphate : oxygen oxidoreductase
Molecular weight: 75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)
Isoelectric point: ~4.2 (230 000 D (Sephadex G 200), 4 x 58 000 D (SDS-gel electrophoresis))
Michaelis constants (L-glycerol phosphate):
K-phosphate buffer, 0.1 mol/L; pH 7.5: 1.36 x 10-2 mol/L (o-dianisidine assay)
Tris buffer, 0.1 mol/L; pH 7.6: 2.90 x 10-3 mol/L (o-dianisidine assay)
Tris buffer, 0.1 mol/L; pH 8.1: 1.40 x 10-3 mol/L (PAP assay)
Structure: Monomeric protein with FAD as cofactor
Inhibitors: Ag, Hg-salts and SDS
pH optimum: 8.0-8.5 (see figure for product Cat. No. 11 654 730 103)
Temperature dependence: See figure for product Cat. No. 11 654 730 103
pH stability: 6.5-8.5 (see figure for product Cat. No. 11 654 730 103)
Thermal stability: See figure for product Cat. No. 11 654 730 103
Specificity: Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.
Appearance: Green-yellow amorphous lyophilizate
Solubility: Clear yellow solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 6.8-7.8
Activity (+25°C, L-α-glycerol phosphate): ≥5 U/mg lyophilizate
Activity (+37°C, L-α-glycerol phosphate): ≥10 U/mg lyophilizate
Specific activity (+25°C): ≥40 U/mg protein
Protein (BCA): ≥0.1 mg/mg lyophilizate
Contaminants (expressed as percentage of Glycerol-3-phosphate Oxidase activity):
Cholesterol oxidase: ≤0.001
Lactate oxidase: ≤0.002
Uricase: ≤0.05
Stability: At +2 to +8°C within specification range for 12 months. Store dry.