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Pyruvate Oxidase

from E.coli overproducer, lyophilizate

For further processing only.

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Order information
Pyruvate Oxidase material number and pack size:
Material Number Pack Size
11418912103 custom fill
Will be supplied as "Pyruvate Oxidase Recombinant (E. coli)". Unit of measure is "kU".
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Overview
Recombinant oxidoreductase that catalyzes the interconversion of pyruvate to acetyl phosphate.
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Application
Use Pyruvate Oxidase in a variety of diagnostic tests, such as for the determination of pyruvate, lactate or aminotransferases.
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Specification
Appearance: Yellow lyophilizate
Solubility: Clear, yellowish solution in potassium phosphate buffer, 0.1 M, pH 6.5 (c=10 mg/mL)
Activity (+25°C, pyruvate, O2, Pi): ≥1.5 U/mg lyophilizate
Specific activity: ≥3 U/mg protein
Protein (Biuret): ≥0.4 mg/mg lyophilizate
Contaminants (expressed as percentage of Pyruvate Oxidase activity):
ATPase: No limit
Glucose oxidase: ≤0.001
Aspartate aminotransferase (AST/GOT): ≤0.01
Alanine aminotransferase (ALT/GPT): ≤0.01
apo-Alanine aminotransferase (apo-ALT/apo-GPT) : ≤0.005
Impurities, total: ≤0.02
Lactate oxidase: ≤0.002
α-Ketoglutarate oxidase: ≤0.02
''NADH oxidase'': ≤0.02
Stability: At -15 to -25°C within specification range for 12 months. Store dry.