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Lactate 2-Monooxygenase (Lactate oxidase), Grade II

from Aerococcus viridans, expressed in E. coli, lyophilizate

For further processing only.

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Order information
Lactate 2-Monooxygenase (Lactate oxidase), Grade II material number and pack size:
Material Number Pack Size
11798197103 custom fill
Will be supplied as "Lactat-OD, rec., Lyo.". Unit of measure is "MU".
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Overview
Recombinant oxidoreductase that catalyzes the conversion of lactate to pyruvate.
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Application
Use Lactate 2-Monooxygenase, Grade II in diagnostic tests for the determination of L-lactate.
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Specification
Nomenclature: L-lactate:oxigen oxidoreductase
Michaelis constant: L-lactate: 5 x 10-4 mol/L
Vmaximum: L-lactate: 0.2 mol/L
pH optimum: 6.5-7.5 (see figure for product Cat. No. 04 822 277 103)
Temperature dependence: See figure for product Cat. No. 04 822 277 103
pH stability: 6.0-9.0 (see figure for product Cat. No. 04 822 277 103)
Thermal stability: Up to +65°C (see figure for product Cat. No. 04 822 277 103)
Appearance: Yellow lyophilizate
Activity (+25°C; with L-lactate) = ≥20 U/mg lyophilized material
Specific activity: ≥55 U/mg protein
Protein (BCA): 0.3-0.7 mg/mg lyophilizate
Contaminants (expressed as percentage of Lactate 2-Monooxygenase activity):
Catalase: ≤0.2
Glucose oxidase: ≤0.001
Pyruvate oxidase: ≤0.001
Uricase: ≤0.001
Stability: At -15 to -25°C within specification range for 12 months. Store dry.