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Hexokinase (HK), chemically modified

from yeast overproducer, lyophilizate

For further processing only.

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Order information
Hexokinase (HK), chemically modified material number and pack size:
Material Number Pack Size
11370600103 custom fill
Will be supplied as "Hexokinase (HK) from Rec.Yeast, Modif.". Unit of measure is "MU".
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Overview
Recombinant enzyme that converts hexose to hexose-6-phosphate.
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Application
Use Hexokinase in diagnostic tests for blood glucose using the hexokinase method and for the determination of creatine kinase. The chemical modification generates an increased liquid stability of the enzyme.
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Specification
Nomenclature: ATP:D-hexose 6-phosphotransferase
Molecular weight: 57 kD (SDS-PAGE)
Isoelectric point: 4.5-5.0
Michaelis constants (D-glucose):
Phosphate buffer, 0.1 mol/L, pH 7.0; +25°C: 3.05 x 10-4 mol/L
Phosphate buffer, 0.1 mol/L, pH 7.4; +30°C: 1.90 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 2.30 x 10-4 mol/L
Michaelis constants (ATP):
Tris buffer, 0.1 mol/L, pH 7.6; +28°C: 1.60 x 10-4 mol/L
Tea buffer, 0.1 mol/L, pH 7.6; +25°C: 1.90 x 10-4 mol/L
Inhibitors: EDTA, SH-blocking compounds, sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxymethylglucose, lyxose.
Activators: Mg2+, catecholamines
pH optimum: 7.0-10.0 (see figure for product Cat. No. 11 119 796 103)
Temperature dependence: See figure for product Cat. No. 11 119 796 103
pH stability: 5.0-9.0 (see figure for product Cat. No. 11 119 796 103)
Thermal stability: See figure for product Cat. No. 11 119 796 103
Specificity: Hexokinase phosphorylates D-glucose, D-fructose, D-mannose, D-glucosamin, 2-deoxyglucose. L-Arabinose, D-xylose, L-rhamnose, D-galactose, D-lactose, sucrose, maltose, trehalose, raffinose, N-acetyl glucosamine do not react. ATP can be partially replaced by other nucleotides.
Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=40 mg/mL)
pH value (c=40 mg/mL in water): 6.5-7.5
Activity (+25°C, glucose): ≥40 U/mg lyophilizate
Contaminants (expressed as percentage of hexokinase activity):
Alcohol dehydrogenase: ≤0.001
ATPase: ≤0.05
Creatine kinase: ≤0.001
G6P-DH: ≤0.005
Glutamate dehydrogenase: ≤0.05
Glutathione reductase: ≤0.005
Myokinase: ≤0.001
''NADH oxidase'': ≤0.001
6-Phosphogluconate dehydrogenase: ≤0.001
Phophoglucose isomerase: ≤0.002
Phosphoglucomutase: ≤0.02
Glucose: ≤0.3 μg/mg lyophilizate
Stability: At +2 to +8°C within specification range for 18 months.
Remark: This enzyme is especially suited for liquid stable applications with extended shelf life requirements.