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Phosphogluconate Dehydrogenase (decarboxylating)

from yeast, lyophilizate

For further processing only.

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Order information
Phosphogluconate Dehydrogenase (decarboxylating) material number and pack size:
Material Number Pack Size
11126482103 custom fill
Will be supplied as "6-PGDH from Yeast, Lyophilizate". Unit of measure is "kU".
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Overview
Dehydrogenase that catalyzes the formation of ribulose 5-phosphate from 6-phosphogluconate.
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Application
Use Phosphogluconate Dehydrogenase in diagnostic tests for the determination of creatine kinase or glucose in the combination with Hexokinase, Catalog Nos. 11 119 796 103, Glucose-6-phosphate Dehydrogenase, Catalog Nos. 10 186 783 103, 11 389 343 103, 11 293 206 103 or 10 190 454 103, and 6-Phosphogluconolactonase, Catalog No. 11 373 129 103.
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Specification
Nomenclature: 6-phospho D-gluconate:NADP+ 2-oxidoreductase (decarboxylating)
Molecular weight: 150 kD (native), 47 kD (SDS-PAGE)
Michaelis constants (TEA, 0.1 mol/L, pH 7.6, +25°C):
6-Phosphogluconate: 7.1 x 10–5 mol/L
NADP: 1.3 x 10-4 mol/L
Inhibitor constant (Phosphate buffer, pH 7.5):
Pyridoxal-5-P: 4.3 x 10–5 mol/L competitive
Inhibitors: Pyridoxal-5-P, iodoacetate and 4-hydroxymercuribenzoate
Activators: Chelators (EDTA, cysteine) plus metal ions (Mg2+); NaCl (0.2 mol/L), KCl (0.2 mol/L).
pH optimum: 7.8 (see figure)
Temperature dependence: See figure
pH stability: 5.0-8.0 (see figure)
Thermal stability: Up to +45°C (see figure)
Specificity: Phosphogluconate dehydrogenase is specific for NADP; NAD does not react.
Appearance: White lyophilizate
Solubility: Clear, colorless solution in water (c=10 mg/mL)
pH value (c=10 mg/mL in water): 7.0-8.0
Protein (Biuret): 0.08-0.16 mg/mg lyophilizate
Activity (+25°C, gluconate-6-P): ≥2 U/mg lyophilizate
Specific activity: ≥12 U/mg protein
Contaminants (expressed as percentage of 6-Phosphogluconate Dehydrogenase activity):
Creatine kinase: ≤0.006
G6P-DH: ≤0.01
Glutathione reductase: ≤0.01
Hexokinase: ≤0.01
Phosphoglucose isomerase: ≤0.03
Stability: At +2 to +8°C within specification range for 12 months.
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Performance
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