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Ascorbate Oxidase

from Cucurbita species, lyophilizate

For further processing only.

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Order information
Ascorbate Oxidase material number and pack size:
Material Number Pack Size
10199605103 custom fill
Will be supplied as ''Ascorbate Oxidase from Cucurbita species''. Unit of measure is ''kU''.
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Overview
Oxidoreductase that oxidizes ascorbic acid to dehydroascorbate.
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Application
Use Ascorbate Oxidase in a variety of diagnostic tests to eliminate the interference of ascorbic acid, since ascorbic acid interferes with the Trinder reaction that is widely used for the colorimetric determination of analytes. It is useful in liquid as well as dry chemistry test, e.g., for the determination of uric acid, lactate or creatinine.
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Specification
Nomenclature: L-ascorbate:oxygen oxidoreductase
Molecular weight: Approximately 140 kD
Isoelectric point: 5.0-6.0
Michaelis constant (Phosphate buffer, pH 5.6, +25°C):
L-ascorbate: 3 x 10-4 mol/L
Inhibitors: 4-chloromercuribenzoate, CN-, Na2S, diethyl-dithiocarbamate, 8-hydroxyquinoline, K-ethylxanthate
pH optimum: 5.6-7.0 (see figure)
Temperature dependence: See figure
pH stability: 6.5-9.0 (see figure)
Thermal stability: Up to +70°C (see figure)
Stability of the lyophilizate: Stable at +35°C for at least 3 weeks (see figure).
Stability in solution: See figure
Specificity: Several analogs of ascorbate react.
Remark: A decrease in activity of approximately 10% may occur.
Appearance: Turquoise lyophilizate
Solubility: Clear, slightly turquoise solution in water (c=50 mg/mL)
pH value (c=50 mg/mL in water): 7.0-8.0
Activity (+25°C, L-ascorbate): ≥170 U/mg lyophilizate
Specific activity (+25°C): ≥1,700 U/mg protein
Protein (Biuret): 0.07-0.14 mg/mg lyophilizate
Contaminants (+25°C; expressed as percentage of Ascorbate Oxidase activity):
Catalase: ≤0.2
Aspartate aminotransferase (AST/GOT): No limit
Alanine aminotransferase (ALT/GPT): No limit
Peroxidase: ≤0.005
Stability: At -15 to -25°C within specification range for 12 months. Keep tightly sealed.
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