Glucose Oxidase (GOD), Grade II
from Aspergillus niger overproducer, lyophilizate
For further processing only.
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Glucose Oxidase (GOD), Grade II material number and pack size: Material Number Pack Size 11939998103 custom fill
- Oxidoreductase that catalyzes the conversion of D-glucose to D-glucono-1,5-lactone which hydrolyzes spontanously to gluconate.
- Use Glucose Oxidase (GOD), Grade II for the determination of α-amylase and D-glucose or O2.
Nomenclature: β-D-glucose:oxygen 1-oxidoreductase
Molecular weight: 79 kD
Isoelectric point: 4.3
Michaelis constants (Glucose):
Acetate buffer, pH 5.0, +25°C: 3.6 x 10-2 mol/L
Potassium phosphate buffer, 0.2 mol/L, pH 7.5, +25°C: 4.8 x 10-2 mol/L
Inhibitors: Ag+, Hg2+, Cu2+, 4-choloromercuribenzoate, D-arabinose (50%). FAD binding is inhibited by several nucleotides.
pH optimum: 7.0 (see figure for product Cat. No. 12 158 566 103 )
Temperature dependence: See figure for product Cat. No. 12 158 566 103
pH stability: See figure for product Cat. No. 12 158 566 103
Thermal stability: See figure for product Cat. No. 12 158 566 103
Specificity: Glucose oxidase is specific for β-D-glucose. O2 can be replaced by hydrogen acceptors such as 2,6-dichlorophenol indophenol. Appearance: Yellow brown lyophilizate
Solubility: Clear, yellow solution in phosphate buffer, 0.1 mol/L, pH 7.0 (c=5 mg/mL)
pH value (c=10 mg/mL in water): 6.8-7.8
Activity (+25°C, glucose): ≥250 U/mg lyophilizate
Protein (Pierce): No limit
Contaminants (expressed as percentage of Glucose Oxidase activity):
Catalase: ≤5 U/mg lyophilizate
Stability: At +2 to +8°C within specification range for 24 months. Store dry.