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β-Galactosidase

from E. coli, lyophilizate

For further processing only.

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Order information
β-Galactosidase material number and pack size:
Material Number Pack Size
11291963103 custom fill
Will be supplied as "β-Galactosidase, Lyo.". Unit of measure is "MU".
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Application
Marker enzyme for the manufacturing of antibody- and antigen-enzyme conjugates incorporated in immunoassays for colorimetric and fluorimetric detection.
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Specification
Nomenclature: β-D-galactohydrolase
Molecular weight (by sequence): 465 kD
Structure: 4 identical subunits; β-galactosidase contains no carbohydrates
Appearance: White lyophilizate
pH value (c=10 mg/mL, in water): 7.0-8.0
Protein (Biuret): 0.25-0.5 mg/mg lyophilizate
Activity (+37°C, 2-NP-β-D-galactoside): ≥120 U/mg lyophilizate
Specific activity (+37°C, 2-nitrophenyl-β-D-galactopyranoside): ≥300 U/mg protein
Contaminants (expressed as percentage of β-Galactosidase activity):
β-Fructosidase: ≤0.001
α-Galactosidase: ≤0.001
Glucose-DH: <0.001
α-Glucosidase: ≤0.001
''NADH oxidase'': ≤0.001
Na (flame photometric): ≤2,500 ppm
Stability: At +2 to +8°C: within specification range for 12 months. Store dry.
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License disclaimer

78:The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.