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FcRn Affinity Column

Prepacked column with immobilized human FcRn receptor for affinity chromatography

For use in quality control / manufacturing process only.

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Order information
FcRn Affinity Column material number and pack size:
Material Number Pack Size
08128057001 1 piece
Unit of measure is "piece".
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Overview
FcRn affinity columns can be used to characterize the binding of therapeutic IgGs to FcRn.
Biotinylated recombinant human FcRn are immobilized to a Streptavidin Sepharose matrix and packed into an FPLC column.
Laborious and time-consuming conventional methods can be replaced.

Instead use FcRn affinity columns for:
  • Superior results
  • Shorter time-to-results
  • Reduced hands-on time
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Application
The column is designed for chromatographic differentiation of structural variants of monoclonal IgGs. FcRn analysis can provide insight into the structural and functional integrity of therapeutic IgG and enables improved conclusions on the half-life of the IgG in vivo:

The neonatal Fc receptor (FcRn) immobilized in this chromatography column has a specific binding affinity to IgG antibodies. The strength of the binding depends on structural variations of the IgG, like different Fab fragments, mutations in the Fc part, oxidized forms, or aggregates. The FcRn affinity chromatography enables differentiation of such variants by peak patterns and retention times in the elution profile.
Furthermore, the FcRn receptor has a binding affinity also to serum albumin, and the FcRn affinity chromatography can also be used to characterize albumin preparations, e.g. for differentiation of oxidized or aggregated variants.

Proteins binding to FcRn:
  • Human IgG1, IgG2, IgG3, IgG4
  • Some IgG of non-human species are able to bind human FcRn
  • Human albumin (often used as a fusion protein for therapeutic proteins)
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Specification
Prepacked chromatography column with approximately 1 mL resin and a binding capacity of ≥ 100 µg IgG, stable for ≥ 100 injections, supplied with standard fittings for liquid chromatography systems (FPLC, HPLC)
Maximal system pressure:  5 bar
Maximal recommended volumetric flow rate:  0.5 mL/minute
HPLC: Corresponds to specification
Stability: At +2 to +8°C within specification for 12 months